Rabbit polyclonal antibody to RFWD2 (phospho Ser387).
Specificity
This antibody detects endogenous levels of RFWD2 only when phosphorylated at Ser387.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:1000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human RFWD2 around the phosphorylation site of Ser387 (amino acids 353-402).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
80kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Constitutive photomorphogenesis protein 1 homolog, COP, E3 ubiquitin-protein ligase RFWD2, hCOP1, Rfwd2, RFWD2_HUMAN, ring finger and WD repeat domain 2, RING finger and WD repeat domain protein 2, RING finger protein 200
Figure 1: Western Blot - Anti-RFWD2 (phospho Ser387) Antibody (A94809)
Western blot analysis of lysates from K562 cells treated with UV 15' using Anti-RFWD2 (phospho Ser387) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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