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Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)

SDS-PAGE - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.com Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.com Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.com Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.com
$630
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$40 to United States
Lead Time
4-6 Business Days

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Name
Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional)
Description
Full-length soluble protein with foldon trimerization motif, mutated Furin recognition site and 2 stabilising mutations (K986P and V987P), based on/modified from Amanat et al, 2020 (PMID: 32398876).
Applications
WB, SDS-PAGE, Immunoassays, Functional Studies
Expression System
HEK293 cells
Protein Species
SARS-CoV-2
Protein Length
Protein fragment
Sequence
VNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGGASASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPSGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGSHHHHHHHH
Amino Acids
16 to 1213
Tag
His Tag (8xHis at the C-terminus).
Predicted MW
137 kDa (including tags).
Biologically Active
Yes
Purity
> 95% (by SDS-PAGE).
Purification
Affinity chromatography.
Product Form
Liquid
Concentration
Lot specific.
Formulation
Supplied in Phosphate Buffered Saline with 20% Glycerol.
Endotoxin Level
< 0.1 ng/µg (1 IEU/µg), as measured by LAL method.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -70°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)
Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) on SDS-PAGE under reducing conditions. The gel was stained with Instant Blue Stain. The purity of the protein is greater than 95%. Estimated molecular weight (SDS-PAGE) of 190 kDa.
Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.comEnlarge Image
Figure 2: Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)
Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) was immobilised on 96-well plates as the capture antigen. Human serum samples from a serological verification panel containing Anti-SARS-CoV-2 Antibodies from National Institute of Biological Standards and Controls, NIBSC (20/B770), were added to the plate for incubation and subsequent washing. Secondary Anti-Human IgG Antibodies (HRP) were added for detection in a sandwich ELISA format. The final colorimetric reaction occurs upon addition of TMB substrate which is quenched by the addition of acid solution.
Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.comEnlarge Image
Figure 3: Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)
Binding curve of Anti-COVID-19 & SARS-CoV S Glycoprotein Antibody [CR3022] to Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional).
Functional Studies - Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172) - Antibodies.comEnlarge Image
Figure 4: Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)
Binding curve of Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional), HRP-conjugated, to Recombinant Human ACE2 Protein (Functional) (A269989) (capture reagent) in direct ELISA assay.
Publishing research using Recombinant SARS-CoV-2 Spike Protein (D614G Variant) (Functional) (A122172)? Please let us know so that we can list the citation on this page.
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