Recombinant SARS-CoV-2 Spike Protein (B.1.617.2 Variant) (Functional) (A270599)

Recombinant SARS-CoV-2 Spike Protein (B.1.617.2 Variant) (Functional)

Full-length soluble protein with foldon trimerization motif, mutated Furin recognition site and 6 stabilising mutations (F817P, A892P, A899P, A942P, K986P and V987P), based on/modified from Amanat et al, 2020 (PMID: 32398876) and Hsieh et al, 2020 (PMID: 32703906).

WB, SDS-PAGE, Immunoassays, Functional Studies

HEK293 cells

SARS-CoV-2

Protein fragment

VNLRTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDVYYHKNNKSWMESGVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGSKPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSRRGAGSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQNVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKGSGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGSGHHHHHHHH

His Tag (8xHis at the C-terminus).

137 kDa (including tags).

Yes

> 95% (by SDS-PAGE).

Affinity chromatography.

Liquid

Lot specific.

Supplied in Phosphate Buffered Saline with 20% Glycerol.

< 0.1 ng/µg (1 IEU/µg), as measured by LAL method.

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -70°C. Avoid freeze / thaw cycles.

This product is for research use only. It is not intended for diagnostic or therapeutic use.