ED50: <2 ng/ml, as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells, corresponding to a specific activity of >5.0 × 105 IU/mg in the presence of 5 ng/ml BMP-4.
Purity
>95% as determined by SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from a solution containing 30% Acetonitrile and 0.1% TFA.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.