Recombinant Mouse Dihydrofolate Reductase (DHFR) Protein (N-terminal His Tag) (A62658)

Recombinant Mouse Dihydrofolate Reductase (DHFR) Protein (N-terminal His Tag)

SDS-PAGE

Escherichia coli

Recombinant

Mouse

Full length protein

MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

N-terminal His Tag

23.8 kDa

>0.2 U/mg.

One unit is defined as the amount of enzyme that will convert 1 µmole of 7,8 -dihydrofloate and beta-NADPH to 5,6,7,8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25°C.

>95% as determined by SDS-PAGE.

Liquid

1mg/ml

Supplied in 20 mM Tris HCl Buffer, pH 8, with 100 mM Sodium Chloride, 2 mM DTT, and 10% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

DHFR, DHFRP1, Dihydrofolate reductase, DYR, DYR_HUMAN, EC 1.5.1.3