ED50: 5-20 µg/ml, as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells.
Purity
>97% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 1 mM DTT and 0.05% Tween 80.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
