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Recombinant Mal d 1 (1.0101) Protein (A242923)

Flow Cytometry - Recombinant Mal d 1 (1.0101) Protein (A242922) - Antibodies.com SDS-PAGE - Recombinant Mal d 1 (1.0101) Protein (A242923) - Antibodies.com ELISA - Recombinant Mal d 1 (1.0101) Protein (A242923) - Antibodies.com
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Name
Recombinant Mal d 1 (1.0101) Protein
Applications
Flow Cytometry, ELISA
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Malus domestica (Apple)
Sequence
DNA sequence encoding 180 amino acids was fused with a Strep Tag at the N-terminus to form this recombinant protein.
Tag
Strep Tag (N-terminus)
Predicted MW
19.9 kDa
Conjugate

Unconjugated

Purification
Ion exchange and affinity chromatography.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 100mM Tris Buffer, pH 8, with 150mM NaCl, and without preservatives or carriers (0.2 µm filter sterilized).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Use immediately or aliquot and store at -20°C to -80°C. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Recombinant Mal d 1 (1.0101) Protein (A242922) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Recombinant Mal d 1 (1.0101) Protein (A242923)
Flow cytometry dot-plot staining pattern of Recombinant Mal d 1 (1.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 [MEM-259] (FITC) Antibody and Anti-CD203c [NP4D6] (PE) Antibody.
SDS-PAGE - Recombinant Mal d 1 (1.0101) Protein (A242923) - Antibodies.comEnlarge Image
Figure 2: SDS-PAGE - Recombinant Mal d 1 (1.0101) Protein (A242923)
Purity verification: 5 µg of Recombinant Mal d 1 (1.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
ELISA - Recombinant Mal d 1 (1.0101) Protein (A242923) - Antibodies.comEnlarge Image
Figure 3: Recombinant Mal d 1 (1.0101) Protein (A242923)
An ELISA test was designed to prove the bond between the coated target, Recombinant Mal d 1 (1.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Malus domestica positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Mal d 1 (1.0101) Protein.
Publishing research using Recombinant Mal d 1 (1.0101) Protein (A242923)? Please let us know so that we can list the citation on this page.
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