Recombinant Listeriolysin (LLO) Protein (A58571)

$300
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Recombinant Listeriolysin (LLO) Protein
Applications
SDS-PAGE
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Listeria monocytogenes
Protein Length
Full length protein
Sequence
MKKIMLVFITLILISLPIAQQTEAKDASAFHKEDLISSMAPPTSPPASPKTPIEKKHADEIDKYIQGLDYNKNNVLVYHGDAVTNVPPRKGYKDGNEYIVVEKKKKSINQNNADIQVVNAISSLTYPGALVKANSELVENQPDVLPVKRDSLTLSIDLPGMTNQDNKIVVKNATKSNVNNAVNTLVERWNEKYAQAYPNVSAKIDYDDEMAYSESQLIAKFGTAFKAVNNSLNVNFGAISEGKMQEEVISFKQIYYNVNVNEPTRPSRFFGKAVTKEQLQALGVNAENPPAYISSVAYGRQVYLKLSTNSHSTKVKAAFDAAVSGKSVSGDVELTNIIKNSSFKAVIYGGSAKDEVQIIDGNLGDLRDILKKGATFNRETPGVPIAYTTNFLKDNELAVIKNNSEYIETTSKAYTDGKINIDHSGGYVAQFNISWDEINYDPEGNEIVQHKNWSENNKSKLAHFTSSIYLPGNARNINVYAKECTGLAWEWWRTVIDDRNLPLVKNRNISIWGTTLYPKYSNSVDNPIE
Predicted MW
58 kDa
Biologically Active
Yes
Biological Activity
Hemolytic activity for Recombinant Listeriolysin O was 1.18 x 105 H U/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay.2mM DTT is used to reactivate the toxin.
Purity
>90% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied at pH 6.4 with 50 mM Sodium Phosphate, 500 mM Sodium Chloride, 2.7 mM Potassium Chloride, 1 mM EDTA, 1 mM DTT, and 5% (v/v) Glycerol.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hly, hlyA, lisA, LLO, Thiol activated cytolysin
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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