Recombinant Human Transferrin Receptor Protein (6xHis Tag)
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
6xHis Tag + TFRC (Cys89-Phe760)
Tag
N-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 76 kDa after removal of the signal peptide. The apparent molecular mass of His-TFRC is approximately 70-100 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 85%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 2: Recombinant Human Transferrin Receptor Protein (6xHis Tag) (A317269)
ELISA plates were pre-coated with Recombinant Human Transferrin Receptor Protein (6×His Tag) (A317269) at 2 µg/ml (100 µl/well) which can bind Pabinafusp Alfa Biosimilar - Anti-Transferrin Receptor Antibody - BSA and Azide free (A318833) in a linear range of 0.13-16 ng/ml.
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