Recombinant Human TNF alpha Protein (C-terminal His Tag) (A61497)

Recombinant Human TNF alpha Protein (C-terminal His Tag)

Cytotoxic Assay

Baculovirus infected Sf9 cells

Recombinant

Human

Protein fragment

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVVPSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYLGGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH

C-terminal His Tag

18.1 kDa

ED50: = 0.2 ng/ml, as determined in a cytotoxicity assay using L929 mouse fibrosarcomacells in the presence of the metabolic inhibitor actinomycin D.

>90% as determined by SDS-PAGE.

Liquid

1mg/ml

Supplied in Phosphate Buffered Saline, pH 7.4, with 10% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form