Recombinant Human TNF alpha Protein (6xHis Tag) (A318337)

Name: Recombinant Human TNF alpha Protein (6xHis Tag)
Brand: Antibodies.com
SKU: A318337
Price: 535.0 USD
Availability: InStock

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$535

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Specifications

Name

Recombinant Human TNF alpha Protein (6xHis Tag)

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment

Sequence

TNFa (Val77-Leu233) + 6xHis Tag

Tag

C-terminal 6xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Recombinant Human TNF alpha Protein (6xHis Tag) (A318337)

ELISA plates were pre-coated with Recombinant Human TNF alpha Protein (6×His Tag) (A318337) at 2 µg/ml (100 µl/well) which can bind Certolizumab Biosimilar - Anti-TNF alpha Antibody - BSA and Azide free (A324624) in a linear range of 0.64–16 ng/ml.

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Figure 2: Recombinant Human TNF alpha Protein (6xHis Tag) (A318337)

ELISA plates were pre-coated with Recombinant Human TNF alpha Protein (6×His Tag) (A318337) at 2 µg/ml (100 µl/well) which can bind Golimumab Biosimilar - Anti-TNF alpha Antibody - BSA and Azide free (A324668) in a linear range of 3.20–80 ng/ml.

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Figure 3: Recombinant Human TNF alpha Protein (6xHis Tag) (A318337)

ELISA plates were pre-coated with Recombinant Human TNF alpha Protein (6×His Tag) (A318337) at 1 µg/ml (100 µl/well) which can bind Adalimumab Biomsimilar - Anti-TNF alpha Antibody - BSA and Azide free (A318901) in a linear range of 0.64-16 ng/ml.

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Figure 4: SDS-PAGE - Recombinant Human TNF alpha Protein (6xHis Tag) (A318337)

SDS-PAGE of Recombinant Human TNF alpha Protein (6×His Tag) (A318337) under reducing conditions.

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