Recombinant Human Syndecan-1 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
CD138 (Gln23-Gly254) + hFc (Glu99-Ala330) + 6xHis Tag
Tag
C-terminal Human Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of CD138-hFc-His is approximately 70-100 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 2: Recombinant Human Syndecan-1 Protein (Fc Chimera 6xHis Tag) (A318402)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human Syndecan-1 Protein (Fc Chimera 6xHis Tag) (A318402) which can bind Anti-CD138 antibody in a linear range of 1.6-200 µg/ml.
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