Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)

Name: Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag)
Brand: Antibodies.com
SKU: A318385
Price: 565.0 USD
Availability: InStock

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$565

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Specifications

Name

Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag)

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment.

Sequence

PD-L1 (Phe19-Arg238) + mFc (Pro99-Lys330) + 6xHis Tag

Tag

C-terminal Mouse Fc Tag and 6xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)

SDS-PAGE of Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) under reducing conditions.

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Figure 2: Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)

ELISA plates were pre-coated with Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) at 2 µg/ml (100 µl/well) which can bind Atezolizumab Biosimilar - Anti-PD-L1 Antibody - BSA and Azide free (A318947) in a linear range of 0.24-7.81 ng/ml.

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Figure 3: Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)

ELISA plates were pre-coated with Recombinant Human PD1 Protein (Fc Chimera 6xHis Tag) (A318264) at 2 µg/ml (100 µl/well) which can bind Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) in a linear range of 62.5-251.1 ng/ml.

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Figure 4: Flow Cytometry - Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385)

Flow cytometry analysis of 15µg/ml of Recombinant Human PD-L1 Protein (Fc Chimera 6xHis Tag) (A318385) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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