Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cellsanswas fount to be 0.04-0.2µg/mL in the presence of 50 ng/ml of Recombinant Human BMP-4.
Purity
>95% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 0.02% Tween 20 and 5% Trehalose.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C. Avoid freeze/thaw cycles.
