Recombinant Human MMP1 Protein (C-terminal His Tag) (A61928)

$155
Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Recombinant Human MMP1 Protein (C-terminal His Tag)
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Tag
C-terminal His Tag
Predicted MW
52 kDa
Biologically Active
Yes
Biological Activity
The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is >400 pmoles/min/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.
Purity
>95% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in MES Buffer with Sodium Chloride, Brij35, and Glycerol.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
Synonyms
27 kDa interstitial collagenase, CLG, CLGN , collagenase, fibroblast, collagenase, interstitial, Fibroblast collagenase, Interstitial collagenase , Matrix metallopeptidase 1, Matrix metallopeptidase 1 (interstitial collagenase), Matrix metalloprotease 1, Matrix Metalloproteinase 1 , Matrix metalloproteinase-1, MMP 1 , MMP-1, MMP1_HUMAN, OTTHUMP00000045866
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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