Recombinant Human M-CSF Protein (Fc Tag) (A318188)

Name: Recombinant Human M-CSF Protein (Fc Tag)
Brand: Antibodies.com
SKU: A318188
Price: 565.0 USD
Availability: InStock

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$565

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Specifications

Name

Recombinant Human M-CSF Protein (Fc Tag)

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment

Sequence

M-CSF (Glu33-Arg255) + hFc (Glu99-Ala330)

Tag

C-terminal Human Fc Tag

Molecular Weight

The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide. The apparent molecular mass of M-CSF-hFc is approximately 55-70 kDa due to glycosylation.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, Macrophage colony stimulating factor, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930, Processed macrophage colony-stimulating factor 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Recombinant Human M-CSF Protein (Fc Tag) (A318188)

SDS-PAGE of Recombinant Human M-CSF Protein (Fc Tag) (A318188) under reducing conditions.

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Figure 2: Recombinant Human M-CSF Protein (Fc Tag) (A318188)

ELISA plates were pre-coated with Recombinant Human CSF-1-R Protein (6×His Tag) (A318346) at 2 µg/ml (100 µl/well) which can bind Recombinant Human M-CSF Protein (Fc Tag) (A318188) in a linear range of 0.32-200 ng/ml.

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Figure 3: Recombinant Human M-CSF Protein (Fc Tag) (A318188)

ELISA plates were pre-coated with Recombinant Human M-CSF Protein (Fc Tag) (A318188) at 2 µg/ml (100 µl/well) which can bind Lacnotuzumab Biosimilar - Anti-M-CSF Antibody - BSA and Azide free (A318861) in a linear range of 9.77-156.25 ng/ml.

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Figure 4: Flow Cytometry - Recombinant Human M-CSF Protein (Fc Tag) (A318188)

Flow cytometry analysis of 1µg/ml of Recombinant Human M-CSF Protein (Fc Tag) (A318188) on Expi293 cells transfected with human CSF1R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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