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Recombinant Human IL-5 Protein (6xHis Tag) (A318331)

SDS-PAGE - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.com Flow Cytometry - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.com ELISA - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.com
$515
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$40 to United States
Lead Time
7-8 Business Days

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Name
Recombinant Human IL-5 Protein (6xHis Tag)
Applications
SDS-PAGE, Flow Cytometry
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
IL5 (Ile20-Ser134) + 6xHis Tag
Tag
C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide. The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Conjugate

Unconjugated

Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor, Colony stimulating factor eosinophil, EDF , Eosinophil differentiation factor, IL 5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin 5 (colony stimulating factor, eosinophil), Interleukin-5, Interleukin5, T cell replacing factor, T-cell replacing factor, TRF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Recombinant Human IL-5 Protein (6xHis Tag) (A318331)
SDS-PAGE of Recombinant Human IL-5 Protein (6×His Tag) (A318331) under reducing conditions.
Flow Cytometry - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Recombinant Human IL-5 Protein (6xHis Tag) (A318331)
Flow cytometry analysis of 1µg/ml of Recombinant Human IL-5 Protein (6×His Tag) (A318331) on Expi293 cells transfected with Human IL5RA protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA - Recombinant Human IL-5 Protein (6×His Tag) (A318331) - Antibodies.comEnlarge Image
Figure 3: Recombinant Human IL-5 Protein (6xHis Tag) (A318331)
ELISA plates were pre-coated with Recombinant Human IL-5 Protein (6×His Tag) (A318331) at 5 µg/ml (100 µl/well) which can bind Recombinant Human IL-5RA Protein (Fc Tag) (A318202) in a linear range of 6.4-4000 ng/ml.
Publishing research using Recombinant Human IL-5 Protein (6xHis Tag) (A318331)? Please let us know so that we can list the citation on this page.

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