0.7 Units. The enzymatic activity of each Heparanase Active Enzyme batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically. Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.
Unit Definition
One unit is defined as 1 µmole of reducing ends of heparan sulfate substrate formed per minute per mg Heparanase Active Enzyme at 37°C
Purity
>95% as determined by RP-HPLC and SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 20 mM Acetate Buffer, pH 5.4, with 750 mM Sodium Chloride.
Storage
Shipped on dry ice. Store at -80°C. Avoid freeze/thaw cycles.
