Recombinant Human Glucose 6 Phosphate Dehydrogenase Protein (N-terminal His Tag) (A62811)

Recombinant Human Glucose 6 Phosphate Dehydrogenase Protein (N-terminal His Tag)

SDS-PAGE

High Five cells

Recombinant

Human

Full length protein

MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

N-terminal His Tag

61.4 kDa

>45 U/ml obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta NADP.

One unit will oxidize 1 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C.

>95% as determined by SDS-PAGE.

Liquid

500 µg/ml

Supplied in 20 mM Tris HCl Buffer, pH 8, with 100 µM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM Sodium Chloride, and 20% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

EC=1.1.1.49, G6PD, G6PD1, G6PDH, G6pdx, G6PD_HUMAN, Glucose 6 phosphate 1 dehydrogenase, Glucose 6 phosphate dehydrogenase, G6PD, Glucose-6-phosphate 1-dehydrogenase, MET19, POS10, Zwf1p