Recombinant Human Fibroblast Activation Protein, alpha Protein (6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment
Sequence
6xHis Tag + FAP (Arg30-Asp760)
Tag
N-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 85.4 kDa after removal of the signal peptide. The apparent molecular mass of His-FAP(30-760) is approximately 100-130 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 2: Recombinant Human Fibroblast Activation Protein, alpha Protein (6xHis Tag) (A318116)
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human Fibroblast Activation Protein alpha Protein (6×His Tag) (A318116) which can bind Anti-FAP Rabbit Monoclonal Antibody in a linear range of 3.2-80 µg/ml.
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