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Recombinant Human CD86 Protein (Fc Tag) (A318254)

SDS-PAGE - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.com ELISA - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.com ELISA - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.com Flow Cytometry - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.com
$565
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$40 to United States
Lead Time
7-8 Business Days

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Name
Recombinant Human CD86 Protein (Fc Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
B7-2 (Leu26-Pro247) + hFc (Glu99-Ala330)
Tag
C-terminal Human Fc Tag
Molecular Weight
The protein has a predicted molecular mass of 51.5 kDa after removal of the signal peptide.
Conjugate

Unconjugated

Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
Activation B7-2 antigen, Activation B7-2 antigen 3, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, B7, B7 2, B7-2, B7.2, B70, B72 antigen, BU63, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD28LG2, CD86 antigen, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2, CD86 molecule, CD86_HUMAN, CLS1, CTLA-4 counter-receptor B7.2, CTLA-4 counter-receptor B7.2 2, 3, Early T-cell costimulatory molecule 1, ETC-1, FUN 1, FUN-1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane glycoprotein, MGC34413, T-lymphocyte activation antigen CD86, TS/A-2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.comEnlarge Image
Figure 1: SDS-PAGE - Recombinant Human CD86 Protein (Fc Tag) (A318254)
SDS-PAGE of Recombinant Human CD86 Protein (Fc Tag) (A318254) under reducing conditions.
ELISA - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.comEnlarge Image
Figure 2: Recombinant Human CD86 Protein (Fc Tag) (A318254)
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) in a linear range of 0.048-2.694 ng/ml.
ELISA - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.comEnlarge Image
Figure 3: Recombinant Human CD86 Protein (Fc Tag) (A318254)
ELISA plates were pre-coated with Recombinant Human CD86 Protein (Fc Tag) (A318254) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD28 Protein (Fc Chimera 6xHis Tag) (A318394) in a linear range of 62.5-4000 ng/ml.
Flow Cytometry - Recombinant Human CD86 Protein (Fc Tag) (A318254) - Antibodies.comEnlarge Image
Figure 4: Flow Cytometry - Recombinant Human CD86 Protein (Fc Tag) (A318254)
HEK293 cell line was transfected with: Irrelevant protein (B) and human CD28 (A). Cells were surface stained with 1µg/ml of Recombinant Human CD86 Protein (Fc Tag) (A318254) followed by Anti-Human IgG Antibody (Alexa 488).
Publishing research using Recombinant Human CD86 Protein (Fc Tag) (A318254)? Please let us know so that we can list the citation on this page.

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