Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)

Name: Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag)
Brand: Antibodies.com
SKU: A318400
Price: 495.0 USD
Availability: InStock

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$40 to United States

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7-8 Business Days

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Specifications

Name

Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag)

Description

This protein is constructed from the following CD47 sequences: (Gln19-Gln141) (Val198-Ala207) (Ser257-Gly268). The mouse Fc tag consists of amino acids Pro99-Lys330.

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment

Sequence

CD47 (Gln19-Gln141) (Val198-Ala207) (Ser257-Gly268) + mFc (Pro99-Lys330) + 6xHis Tag

Tag

C-terminal Mouse Fc Tag and 6xHis Tag

Molecular Weight

The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

Antigen identified by monoclonal, Antigenic surface determinant protein OA3, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)

SDS-PAGE of Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) under reducing conditions.

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Figure 2: Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)

ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 2 µg/ml (100 µl/well) which can bind Recombinant Human SIRP alpha Protein (Fc Chimera 6xHis Tag) (A318399) in a linear range of 3.3-26.37 ng/ml.

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Figure 3: Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)

ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 2 µg/ml (100 µl/well) which can bind Magrolimab Biosimilar - Anti-CD47 Antibody - BSA and Azide free (A318955) in a linear range of 0.061-1.606 ng/ml.

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Figure 4: Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400)

ELISA plates were pre-coated with Recombinant Human CD47 Protein (Fc Chimera 6xHis Tag) (A318400) at 1 µg/ml (100 µl/well) which can bind Magrolimab Biosimilar - Anti-CD47 Antibody - BSA and Azide free (A318907) in a linear range of 3.2-80.0 ng/ml.

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