The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD 30, CD30 antigen, CD30L receptor, Cytokine receptor CD30, D1S166E, KI 1, KI 1 antigen, Ki-1 antigen, KI1, Lymphocyte activation antigen CD30, TNFRSF 8, Tnfrsf8, TNR8_HUMAN, Tumor necrosis factor receptor superfamily member 8
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Figure 2: Recombinant Human CD30 Protein (6xHis Tag) (A318247)
ELISA plates were pre-coated with Recombinant Human CD30 Protein (6×His Tag) (A318247) at 2 µg/ml (100 µl/well) which can bind Brentuximab Biosimilar - Anti-CD30 Antibody - BSA and Azide free (A318939) in a linear range of 0.13-80.0 ng/ml.
Figure 3: Recombinant Human CD30 Protein (6xHis Tag) (A318247)
ELISA plates were pre-coated with Recombinant Human CD30 Protein (6×His Tag) (A318247) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD30-L Protein (Fc Tag & 6×His Tag) (A318369) in a linear range of 2.56-320 ng/ml.
Figure 4: Flow Cytometry - Recombinant Human CD30 Protein (6xHis Tag) (A318247)
Flow cytometry analysis of 1µg/ml of Recombinant Human CD30 Protein (6×His Tag) (A318247) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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