Recombinant Human CD27 Protein (Fc Tag) (A318253)

Name: Recombinant Human CD27 Protein (Fc Tag)
Brand: Antibodies.com
SKU: A318253
Price: 565.0 USD
Availability: InStock

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$565

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Specifications

Name

Recombinant Human CD27 Protein (Fc Tag)

Applications

ELISA, SDS-PAGE

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Protein Length

Protein fragment.

Sequence

CD27 (Ala20-Arg191) + hFc (Glu99-Ala330)

Tag

C-terminal Human Fc Tag

Molecular Weight

The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. The apparent molecular mass of CD27-hFc is approximately 55-70 kDa due to glycosylation.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

CD 27, CD27 antigen, CD27 molecule, CD27L receptor, CD27_HUMAN, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Recombinant Human CD27 Protein (Fc Tag) (A318253)

SDS-PAGE of Recombinant Human CD27 Protein (Fc Tag) (A318253) under reducing conditions.

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Figure 2: Recombinant Human CD27 Protein (Fc Tag) (A318253)

ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938) in a linear range of 3.2-80.0 ng/ml. In order to specifically detect Varlilumab Biosimilar - Anti-CD27 Antibody - BSA and Azide free (A318938), Mouse Anti-Human Fab Antibody was used as detection antibody.

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Figure 3: Recombinant Human CD27 Protein (Fc Tag) (A318253)

ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD70 Protein (Fc Tag & 6×His Tag) (A318368) in a linear range of 3.2-400 ng/ml.

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Figure 4: Flow Cytometry - Recombinant Human CD27 Protein (Fc Tag) (A318253)

Flow cytometry analysis of 15µg/ml of Recombinant Human CD27 Protein (Fc Tag) (A318253) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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