The activity is determined by the dose-dependent proliferation of the SH-SY5Y cell line and is typically 4-8 ng/ml. The activity can also be determined by its ability to promote survival and neurite outgrowth.
Purity
>98% as determined by RP-HPLC and SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 10 mM Sodium Citrate Buffer, pH 4.5, with 25 mM Sodium Chloride.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
