Recombinant Human Apolipoprotein E Protein (6xHis Tag)
Applications
SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
APOE (Lys19-His317) + 6xHis tag
Tag
C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 35.1 kDa after removal of the signal peptide. The apparent molecular mass of APOE-His is approximately 25-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 85%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 1: SDS-PAGE - Recombinant Human Apolipoprotein E Protein (6xHis Tag) (A324930)
SDS-PAGE of Recombinant Human Apolipoprotein E Protein (6xHis Tag) (A324930) under reducing conditions.
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