Recombinant Firefly Luciferase Protein (N-terminal His Tag) (A113328)

Recombinant Firefly Luciferase Protein (N-terminal His Tag)

SDS-PAGE

Escherichia coli

Recombinant

Common Eastern Firefly

Protein fragment

MGSSHHHHHH SSGLVPRGSH MGSHMTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ

N-terminal His Tag

38.5 kDa

>1x109 light U/mg.

One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25°C.

>95% as determined by SDS-PAGE.

Liquid

0.5mg/ml

Supplied in 20 mM Tris HCl Buffer, pH 8, with 1 mM DTT and 10% Glycerol.

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

ec 1.13.12.7, Firefly, Luciferase, Luciferin 4 monooxygenase