Recombinant Canine PD1 Protein (Fc Tag) (A324899)

Name: Recombinant Canine PD1 Protein (Fc Tag)
Brand: Antibodies.com
SKU: A324899
Price: 660.0 USD
Availability: InStock

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$660

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Freight Charges

$40 to United States

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7-8 Business Days

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Specifications

Name

Recombinant Canine PD1 Protein (Fc Tag)

Applications

SDS-PAGE, ELISA, Flow Cytometry

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Canine

Protein Length

Protein fragment.

Sequence

PD-1 (Leu25-Gly168) + mFc (Pro99-Lys330)

Tag

C-terminal Mouse Fc Tag

Molecular Weight

The protein has a predicted molecular mass of 42.4 kDa after removal of the signal peptide. The apparent molecular mass of dPD-1-mFc is approximately 55-70 kDa due to glycosylation.

Conjugate

Unconjugated

Purity

> 95%, by SDS-PAGE and Coomassie blue staining.

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

CD279, CD279 antigen, hPD 1, hPD l, hPD-1, hSLE1, PD 1, PD-1, PDCD 1, PDCD1, PDCD1_HUMAN, Programmed cell death 1, Programmed cell death 1 protein, Programmed cell death protein 1, Protein PD 1, Protein PD-1, SLEB2, Systemic lupus erythematosus susceptibility 2

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Recombinant Canine PD1 Protein (Fc Tag) (A324899)

SDS-PAGE of Recombinant Canine PD1 Protein (Fc Tag) (A324899) under reducing conditions.

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Figure 2: Recombinant Canine PD1 Protein (Fc Tag) (A324899)

ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324899) at 2 µg/ml (100 µl/well) which can bind INTERVET 4F12 Biosimilar - Anti-PD1 Antibody - BSA and Azide free (A324674) in a linear range of 0.64-80 ng/ml.

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Figure 3: Recombinant Canine PD1 Protein (Fc Tag) (A324899)

ELISA plates were pre-coated with Recombinant Canine PD1 Protein (Fc Tag) (A324899) at 2 µg/ml (100 µL/well) which can bind Recombinant Canine PD-L1 Protein (Fc Tag) (A324699) in a linear range of 16-2000 ng/ml.

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Figure 4: Flow Cytometry - Recombinant Canine PD1 Protein (Fc Tag) (A324899)

Flow cytometry analysis of 1µg/ml of Recombinant Canine PD1 Protein (Fc Tag) (A324899) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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