Recombinant Bet v 2 (2.0101) Protein (A242917)

Purity verification: 5 µg of Recombinant Bet v 2 (2.0101) Protein with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Flow cytometry dot-plot staining pattern of Recombinant Bet v 2 (2.0101) Protein stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using Anti-CD63 Antibody [MEM-259] (FITC) and Anti-CD203c Antibody [NP4D6] (PE).

An ELISA test was designed to prove the bond between the coated target, Recombinant Bet v 2 (2.0101) Protein, and allergen-specific human plasma IgG4 antibodies of Betula verrucosa positive donor. A measurable signal was subsequently generated by the addition of Anti-Human IgG4 Antibody (Biotin), Streptavidin-HRP, and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Recombinant Bet v 2 (2.0101) Protein.