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Anti-RDH5 Antibody (A10279)

Western Blot - Anti-RDH5 Antibody (A10279) - Antibodies.com Immunofluorescence - Anti-RDH5 Antibody (A10279) - Antibodies.com Immunofluorescence - Anti-RDH5 Antibody (A10279) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to RDH5 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-RDH5 Antibody
Description
Rabbit polyclonal antibody to RDH5.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
Sequence
LPASNAFVFITGCDSGFGRLLALQLDQRGFRVLASCLTPSGAEDLQRVASSRLHTTLLDITDPQSVQQAAKWVEMHVKEAGLFGLVNNAGVAGIIGPTPWLTRDDFQRVLNVNTMGPIGVTLALLPLLQQARGRVINITSVLGRLAANGGGYCVSKFGLEAFSDSLRRDVAHFGIRVSIVEPGFFRTPVTNLESLEKTLQACWARLPPATQAHYGGAFLTKYLKMQQRIMNLICDPDLTKVSRCLEHALTARHPRTRYSPGWDAKLLWLPASYLPASLVDAVLTWVLPKPAQAVY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
38 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
11 cis RDH, 11 cis retinol dehydrogenase, 9 cis retinol specific dehydrogenase, FLJ39337, FLJ97089, HSD17B9, RDH 5, RDH1, Retinol dehydrogenase 1, Retinol dehydrogenase 5, Retinol dehydrogenase 5 (11 cis/9 cis), SDR9C5, Short chain dehydrogenase/reductase family 9C member 5
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RDH5 Antibody (A10279) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RDH5 Antibody (A10279)
Western blot analysis of extracts of various cell lines, using Anti-RDH5 Antibody (A10279) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-RDH5 Antibody (A10279) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-RDH5 Antibody (A10279)
Immunofluorescence analysis of rat retina using Anti-RDH5 Antibody (A10279) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-RDH5 Antibody (A10279) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-RDH5 Antibody (A10279)
Immunofluorescence analysis of mouse retina using Anti-RDH5 Antibody (A10279) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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