This TNF alpha enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TNF alpha Antibody. Standards or samples are then added to the microtire plate wells and TNF alpha, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TNF alpha present in the sample, Anti-TNF alpha Antibody (Biotin) is added to each well to "sandwich" the TNF alpha immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF alpha will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 1 pg/ml

Product Range

15.6-1000 pg/ml (body fluids, tissue lysates, and cell culture supernatants) or 7.8-500 pg/ml (serum and plasma).

Reactivity

Rat

Recovery

Storage

Store at 2-8°C for 6 months or at -20°C for 12 months.

Synonyms

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.