Rat PDGF AA ELISA Kit (A327027)

Rat PDGF AA ELISA Kit

Rat PDGF AA ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat PDGF AA in serum, plasma, and other biological fluids.

Sandwich (quantitative)

Rat PDGF AA ELISA Kit (A327027) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat PDGF AA in serum, plasma, and other biological fluids. An antibody specific for PDGF AA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PDGF AA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PDGF AA Antibody, which binds the captured PDGF AA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PDGF AA captured in each well. The concentration of PDGF AA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colormetric

Colorimetric Microplate Reader

Serum, plasma, and other biological fluids.

0.47 pg/ml

0.78-50 pg/ml

Rat

No significant cross-reactivity or interference between rat PDGF AA and analogues was observed.

Intra-assay CV: 5.67%, Inter-assay CV: 4.77%

Information online is representative. Always refer to the Product Manual inside the kit.

Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.

PDGF A, PDGF A chain, PDGF subunit A, PDGF-1, PDGF1, PDGFA, PDGFA_HUMAN, Platelet derived growth factor alpha chain, Platelet derived growth factor alpha isoform 2 preproprotein, Platelet derived growth factor alpha polypeptide, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Platelet-derived growth factor subunit A