This NT-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-NT-4 Antibody. Standards or samples are then added to the microtire plate wells and NT-4, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of NT-4 present in the sample, Anti-NT-4 Antibody (HRP) is added to each well to "sandwich" the NT-4 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NT-4 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, urine, cell culture supernatants, and tissue samples.

Product Range

600-20,000 pg/ml

Reactivity

Rat

Recovery

Storage

Store at 2-8°C for 6 months.

Synonyms

GLC10, GLC1O, Neurotrophic factor 4, Neurotrophic factor 5, Neurotrophin 4, Neurotrophin 4/5, Neurotrophin 5, Neurotrophin 5 (neurotrophin 4/5), Neurotrophin-4, Neurotrophin-5, Neutrophic factor 4, Neutrophic factor 5, NT 4, NT 4/5, NT 5, NT-5, NT4, NT4/5, NT4P, NT5, NTF4, NTF4_HUMAN, NTF5

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.