This MMP2 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-MMP2 Antibody. Standards or samples are then added to the microtire plate wells and MMP2, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of MMP2 present in the sample, Anti-MMP2 Antibody (Biotin) is added to each well to "sandwich" the MMP2 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP2 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Platform

Microplate

Detection Type

Colorimetric

Sample Type

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivity

< 10 pg/ml

Product Range

625-40,000 pg/ml

Reactivity

Rat

Recovery

Storage

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, 72kD type IV collagenase, CLG 4, CLG 4A , CLG4, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, EC 3.4.24.24, Gelatinase A, Gelatinase alpha , Gelatinase neutrophil , Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix metalloproteinase 2, Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase), Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix metalloproteinase II, Matrix metalloproteinase-2, MMP 2, MMP II, MMP-2, MMP2_HUMAN, MONA, Neutrophil gelatinase, PEX, TBE 1, TBE-1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.