Rat CamKII gamma ELISA Kit (A2635)

Rat CamKII gamma ELISA Kit

Rat CamKII gamma ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat CamKII gamma in tissue homogenates, cell lysates or other biological fluids.

Sandwich (quantitative)

Rat CamKII gamma ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of rat CamKII gamma in rat. An antibody specific for CamKII gamma has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CamKII gamma present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CamKII gamma Antibody, which binds the captured CamKII gamma present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CamKII gamma captured in each well. The concentration of CamKII gamma can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

Tissue homogenates, cell lysates or other biological fluids.

0.123 ng/ml

1 - 4.5 Hours

Rat

Intra-assay: CV < 10%, Inter-assay: CV < 12%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

CaM kinase II, CaM kinase II gamma chain, CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CAMKII