Rat Adiponectin ELISA Kit (A327156)

$582
Freight Charges
Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Rat Adiponectin ELISA Kit
Description
Rat Adiponectin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat Adiponectin in serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Rat Adiponectin ELISA Kit (A327156) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Adiponectin in serum and plasma. An antibody specific for Adiponectin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Adiponectin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Adiponectin Antibody, which binds the captured Adiponectin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Adiponectin captured in each well. The concentration of Adiponectin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum and plasma.
Sensitivity
6.43 ng/ml
Product Range
31.25-1000 ng/ml
Assay Time
1h 30m
Reactivity
Rat
Cross Reactivity
No significant cross-reactivity or interference between rat Adiponectin and analogues was observed.
Recovery
Precision
Intra-assay CV: 4.6%, Inter-assay CV: 5.31%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, adipocyte-specific secretory protein, Adiponectin precursor, adiponectin, C1Q and collagen domain containing, Adipoq, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPO_HUMAN, ADIPQTL1, ADPN, APM 1, apM-1, APM1, C1q and collagen domain-containing protein, GBP28, Gelatin binding protein , Gelatin binding protein 28, Gelatin-binding protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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