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Anti-RAP1GAP Antibody [ARC2058] (A307431)

Western Blot - Anti-RAP1GAP Antibody [ARC2058] (A307431) - Antibodies.com Immunofluorescence - Anti-RAP1GAP Antibody [ARC2058] (A307431) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2058] antibody to RAP1GAP for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-RAP1GAP Antibody [ARC2058]
Description
Rabbit monoclonal [ARC2058] antibody to RAP1GAP.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736).
Sequence
MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHF
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2058
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase activating protein 1, Rap1 GTPase-activating protein 1, RAP1GA1, Rap1ga1 protein, Rap1GAP1, RAP1GAPII, RAPGAP, RPGP1_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RAP1GAP Antibody [ARC2058] (A307431) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RAP1GAP Antibody [ARC2058] (A307431)
Western blot analysis of extracts of various cell lines, using Anti-RAP1GAP Antibody [ARC2058] (A307431) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence - Anti-RAP1GAP Antibody [ARC2058] (A307431) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-RAP1GAP Antibody [ARC2058] (A307431)
Immunofluorescence analysis of NIH-3T3 cells using Anti-RAP1GAP Antibody [ARC2058] (A307431) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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