Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
GTP-binding protein smg p21B, K-REV, OK/SW-cl.11, RAL1B, RAP1B member of RAS oncogene family, RAP1B_HUMAN, Ras family small GTP binding protein RAP1B, RAS related protein RAP1B, Ras-related protein Rap-1b, Small GTP binding protein
Figure 1: Western Blot - Anti-RAP1B Antibody (A88743)
Western blot analysis of extracts of various cell lines, using Anti-RAP1B Antibody (A88743) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Anti-RAP1B Antibody (A88743) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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