Anti-RAP1A Antibody (A12733)

Name: Anti-RAP1A Antibody
Brand: Antibodies.com
SKU: A12733
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to RAP1A for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-RAP1A Antibody

Description

Rabbit polyclonal antibody to RAP1A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rap1A (NP_002875.1).

Sequence

QCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C21KG, G 22K, G-22K, GTP binding protein smg p21A, GTP-binding protein smg p21A, KREV 1, KREV1, OTTHUMP00000013741, RAP 1A, RAP1, RAP1A member of RAS oncogene family, RAP1A_HUMAN, Ras related protein Krev 1, Ras related protein Rap 1A, RAS related protein RAP1A, Ras-related protein Krev-1, Ras-related protein Rap-1A, SMGP21

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RAP1A Antibody (A12733)

Western blot analysis of extracts of various cell lines, using Anti-RAP1A Antibody (A12733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-RAP1A Antibody (A12733)

Western blot analysis of extracts from wild type(WT) and Rap1A knockdown (KD) 293T cells, using Anti-RAP1A Antibody (A12733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-RAP1A Antibody (A12733)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-RAP1A Antibody (A12733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-RAP1A Antibody (A12733)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-RAP1A Antibody (A12733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.