Anti-RANKL Antibody - BSA and Azide free (A283063)

$645

Rabbit polyclonal antibody to RANKL for WB, ELISA and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-RANKL Antibody - BSA and Azide free
Description
Rabbit polyclonal antibody to RANKL.
Specificity
This antibody recognises the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.
Applications
WB, ELISA, IHC-P
Dilutions
ELISA: 0.5 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human CD254.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Concentration
1 mg/ml after reconstitution.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human CD254 antibody recognizes the soluble form of human CD254, otherwise known as sRANKL, a ~20 kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions. CD254 is also involved in apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.
Synonyms
CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa B ligand, Receptor activator of nuclear factor kappa-B ligand, sOdf, TNF related activation induced cytokine, TNF-related activation-induced cytokine, TNF11_HUMAN, TNFSF 11, Tnfsf11, TRANCE, Tumor necrosis factor (ligand) superfamily member 11, Tumor necrosis factor ligand superfamily member 11, Tumor necrosis factor ligand superfamily member 11, soluble form
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-RANKL Antibody - BSA and Azide free (A283063)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-RANKL Antibody - BSA and Azide free (A283063)

Top