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Anti-Rag A Antibody (A398)

Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution. Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilution Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on right
$510

Rabbit polyclonal antibody to Rag A for WB.

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4-6 Business Days

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Name
Anti-Rag A Antibody
Description
Rabbit polyclonal antibody to Rag A.
Applications
WB
Dilutions
WB: 1:1,000-1:2,000
Reactivity
Human, Mouse, Rat, Hamster, Xenopus
Immunogen
Purified full length human Rag A protein (GST tag).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Product Form
Liquid
Formulation
Supplied as antiserum with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RagA, Ras-related GTP-binding protein A, RRAGA, RRAGA_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot analysis of RagA protein in the whole cell extracts (HeLa cell g). Anti-RagA antiserum was used at 1,000 dilution.Enlarge Image
Figure 1: Anti-Rag A Antibody (A398)
Anti-Rag A Antibody
Western blot analysis of RagA protein in the whole cell extracts. 1. HeLa (humans) 2. MCF7 (human) 3. MOLT4 (human) 4. NIH3T3 (mouse) Proteins in the extracts (20 µg) were separated on 12.5% SDS-PAGE. Anti-RagA antibody was used at 1/1,000 dilution?Anti-rabbit IgG antibody conjugated with HRP (Abcam;ab97051) was used as the 2nd antibody at 1/10,000 dilutionEnlarge Image
Figure 2: SDS-PAGE - Anti-Rag A Antibody (A398)
Anti-Rag A Antibody
Immunofluorescence staining of RagA protein in HeLa cells with anti-Rag antibody. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution (A) or at 1/1,000 dilution (B). As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 was used at 1/1,000 dilution. DNA was stained with DAPI and the merged image was shown on rightEnlarge Image
Figure 3: Immunofluorescence - Anti-Rag A Antibody (A398)
Anti-Rag A Antibody
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