Anti-Raf1 (phospho Ser259) Antibody (A10977)

Name: Anti-Raf1 (phospho Ser259) Antibody
Brand: Antibodies.com
SKU: A10977
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Raf1 (phospho Ser259) for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Raf1 (phospho Ser259) Antibody

Description

Rabbit polyclonal antibody to Raf1 (phospho Ser259).

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:50-1:100

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1).

Sequence

STSTP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, cRaf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1_HUMAN, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Raf1 (phospho Ser259) Antibody (A10977)

Western blot analysis of extracts of HeLa cells, using Anti-Raf1 (phospho Ser259) Antibody (A10977) at 1:1,000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Raf1 (phospho Ser259) Antibody (A10977)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Raf1 (phospho Ser259) Antibody (A10977). This Western blot was performed on the immunoprecipitate using Anti-Raf1 (phospho Ser259) Antibody (A10977) at a dilution of 1:1000. HeLa cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight.

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