Anti-Raf1 Antibody (A12552)

Name: Anti-Raf1 Antibody
Brand: Antibodies.com
SKU: A12552
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Raf1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Raf1 Antibody

Description

Rabbit polyclonal antibody to Raf1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human Raf1 (NP_002871.1).

Sequence

IRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, cRaf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1_HUMAN, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Raf1 Antibody (A12552)

Western blot analysis of extracts of Mouse liver, using Anti-Raf1 Antibody (A12552) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Raf1 Antibody (A12552)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Raf1 Antibody (A12552) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Raf1 Antibody (A12552)

Immunofluorescence analysis of HeLa cells using Anti-Raf1 Antibody (A12552) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Raf1 Antibody (A12552)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Raf1 Antibody (A12552) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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