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Anti-Rad52 Antibody (A101677)

Western Blot of Rad52 in mammalian cells. M: Marker proteins. Lane 1: Recombinant human Rad52 protein (0.2ng). Lane 2: HeLa cell extrat (17µg). Lane 3: MCF7 cell extract (27µg). Lane 4: NIH3T3 cell extract (20µg). Lane 5: CHO cell extract (21µg). The samples were separated by electrophoresis on 10% SDS-PAGE and blotted onto PVDF membrane. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Molecular mass of human Rad52 protein is 47 kDa. Western blot of Rad52 in Rat cells. Lane 1: Human MCF-7 cell lysate (16µg). Lane 2: Rat PC-12 cell (40µg). Proteins in the lysates were separated on SDS-PAGE (12.5%) and electro-blotted to PVDF membrane overnight. The blot was blocked with 5% skim milk. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Immunoprecipitation of Rad52 from human cell lysate. 1. Add 10µg of Anti-Rad52 Antibody and 50µg of Dynabeads protein A. 2. Incubate for 10 min at room temperature, wash and resuspend in 100µl of the same buffer. 3. Add 100µl (300µg in PBS) lysate of human MCF7 cells. 4. Incubate for and collect the immunocomplex with Dynabeeds magnetic apparatus. 5. Elute the immunocomplex and analyze the presence of Rad52 in the supernatant (Lane 2) and the eluate (Lane 1) by western blotting with Anti-Rad52 Antibody. 6. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 3 is western blot of MCF’ lysate (16µg). HU-induced focus formation of Rad52. U2OS 40-2-6 cells were untreated or treated with 2mM hydroxyurea (HU) for 24 h. The cells were pre-extracted with 0.1% TritonX-100 in PBS, fixed with 3.7% formaldehyde in PBS, and immunostained with Anti-Rad52 Antibody (green) at 1:50 dilution, followed by DAPI staining (blue). Goat Anti-Rabbit IgG Antibody (CF488A) was used as a secondary at 1:1,000 dilution. TritonX-100 treatment extracts chromatin-free Rad52 from nuclei. Scale bars, 20 µm. Detection of Ra52 protein in human fibroblast cell line, GM0637 by immunofluorescence staining. Cells were fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with solution containing 1% SDS and 0.5% Triton for 10 min, washed 3 times with PBS for 3 min, incubated with Anti-Rad52 Antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with DAPI for 1 min and mounted. Photographed with confocal microscope. Anti-Rad52 Antibody was used at 1:1,000 dilution. Goat Anti-Rabbit IgG Antibody (Alexa 488) was used at 1:2,000 dilution. Detection of Rad52 protein in HeLa cells and MCF7 cells by immunofluorescence staining. Fixed with 4% paraformaldehyde, and permeabilized with 0.25% TritonX-100. DNA was stained with DAPI. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (Alexa 488) was used as a secondary at 1:1,000 dilution. ELISA of human Rad52 protein with Anti-Rad52 Antibody. ELISA analysis of human Rad52 protein, using the Anti-Rad52 Antibody. Plate was coated with 100µl of recombinant human Rad52 protein at 1µg/ml per well and 100µl of Anti-Rad52 Antibody at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG Antibody (HRP) was added as a secondary antibody. Color was developed with TMB as substrate.
$475

Rabbit polyclonal antibody to Rad52 for WB, IP, IF and ELISA.

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$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Rad52 Antibody
Description
Rabbit polyclonal antibody to Rad52.
Applications
WB, IP, IF, ELISA
Dilutions
WB: 1:1,000-1:3,000, IP: 1:50-1:1,000, IF: 1:1,000
Reactivity
Human, Mouse, Rat, Hamster
Immunogen
Recombinant full length human Rad52 protein.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in 20mM Phosphate Buffered Saline, pH 7.5, with 0.15M Sodium Chloride and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA repair protein RAD52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog, RAD52 homolog (S. cerevisiae), RAD52_HUMAN, Recombination protein RAD52, Rhabdomyosarcoma antigen MU RMS 40.23
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot of Rad52 in mammalian cells. M: Marker proteins. Lane 1: Recombinant human Rad52 protein (0.2ng). Lane 2: HeLa cell extrat (17µg). Lane 3: MCF7 cell extract (27µg). Lane 4: NIH3T3 cell extract (20µg). Lane 5: CHO cell extract (21µg). The samples were separated by electrophoresis on 10% SDS-PAGE and blotted onto PVDF membrane. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Molecular mass of human Rad52 protein is 47 kDa.Enlarge Image
Figure 1: SDS-PAGE - Anti-Rad52 Antibody (A101677)
Anti-Rad52 Antibody
Western blot of Rad52 in Rat cells. Lane 1: Human MCF-7 cell lysate (16µg). Lane 2: Rat PC-12 cell (40µg). Proteins in the lysates were separated on SDS-PAGE (12.5%) and electro-blotted to PVDF membrane overnight. The blot was blocked with 5% skim milk. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution.Enlarge Image
Figure 2: SDS-PAGE - Anti-Rad52 Antibody (A101677)
Anti-Rad52 Antibody
Immunoprecipitation of Rad52 from human cell lysate. 1. Add 10µg of Anti-Rad52 Antibody and 50µg of Dynabeads protein A. 2. Incubate for 10 min at room temperature, wash and resuspend in 100µl of the same buffer. 3. Add 100µl (300µg in PBS) lysate of human MCF7 cells. 4. Incubate for and collect the immunocomplex with Dynabeeds magnetic apparatus. 5. Elute the immunocomplex and analyze the presence of Rad52 in the supernatant (Lane 2) and the eluate (Lane 1) by western blotting with Anti-Rad52 Antibody. 6. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 3 is western blot of MCF’ lysate (16µg).Enlarge Image
Figure 3: Immunoprecipitation - Anti-Rad52 Antibody (A101677)
Anti-Rad52 Antibody
HU-induced focus formation of Rad52. U2OS 40-2-6 cells were untreated or treated with 2mM hydroxyurea (HU) for 24 h. The cells were pre-extracted with 0.1% TritonX-100 in PBS, fixed with 3.7% formaldehyde in PBS, and immunostained with Anti-Rad52 Antibody (green) at 1:50 dilution, followed by DAPI staining (blue). Goat Anti-Rabbit IgG Antibody (CF488A) was used as a secondary at 1:1,000 dilution. TritonX-100 treatment extracts chromatin-free Rad52 from nuclei. Scale bars, 20 µm.Enlarge Image
Figure 4: Anti-Rad52 Antibody (A101677)
Anti-Rad52 Antibody
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