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Anti-Rad18 Antibody (A353)

Indirect immunofluorescence staining of Rad18 protein in GM637 cells. Rad18 protein is stained as yellow dots in nuclei. Identification of Rad18 protein in crude extract of A549 cells by western blotting with Anti-Rad18 Antibody. The primary and secondary antibodies are used at 1:1,000 and 1:20,000 dilutions, respectively. The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18. Colocalization of Rad18 with PCNA on chromatin following UV irradiation. UV-induced colocalization of Rad18 (green) with PCNA (red). GM637 cells irradiated at 15/m2 were fixed with methnol 4h after UV irradiation and immunostained with Anti-Rad18 Antibody and Anti-PCNA Antibody and processed for double staining. Anti-Rad18 Antibody was used at 1:250 dilution and Goat Anti-Rabbit IgG Antibody (Alexa Fluor 488) was used as a secondary at 4µg/ml.
$535

Rabbit polyclonal antibody to Rad18 for WB, IP and IF.

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$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Rad18 Antibody
Description
Rabbit polyclonal antibody to Rad18.
Applications
WB, IP, IF
Dilutions
WB: 1:1,000
Reactivity
Human
Immunogen
Synthetic peptide containing 113 carboxyl terminal amino acids of human Rad18 protein (GST tag).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2810024C04Rik, DNA repair protein rad18, E3 ubiquitin-protein ligase RAD18, EC 6.3.2.-, hHR 18, hHR18, hRAD 18, hRAD18, MGC156682, Postreplication repair E3 ubiquitin-protein ligase RAD18, Postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD 18, RAD18 homolog, RAD18 homolog (S. cerevisiae), RAD18 S. cerevisiae homolog, RAD18 S. cerevisiae homolog of, RAD18 transcript variant, Rad18sc, RAD18_HUMAN, Radiation sensitivity protein 18, RING finger protein 73, RNF 73, RNF73, Structural maintenance of chromosomes protein 6, YCR066W, YCR66W
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Indirect immunofluorescence staining of Rad18 protein in GM637 cells. Rad18 protein is stained as yellow dots in nuclei.Enlarge Image
Figure 1: Anti-Rad18 Antibody (A353)
Anti-Rad18 Antibody
Identification of Rad18 protein in crude extract of A549 cells by western blotting with Anti-Rad18 Antibody. The primary and secondary antibodies are used at 1:1,000 and 1:20,000 dilutions, respectively. The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18.Enlarge Image
Figure 2: Anti-Rad18 Antibody (A353)
Anti-Rad18 Antibody
Colocalization of Rad18 with PCNA on chromatin following UV irradiation. UV-induced colocalization of Rad18 (green) with PCNA (red). GM637 cells irradiated at 15/m2 were fixed with methnol 4h after UV irradiation and immunostained with Anti-Rad18 Antibody and Anti-PCNA Antibody and processed for double staining. Anti-Rad18 Antibody was used at 1:250 dilution and Goat Anti-Rabbit IgG Antibody (Alexa Fluor 488) was used as a secondary at 4µg/ml.Enlarge Image
Figure 3: Anti-Rad18 Antibody (A353)
Anti-Rad18 Antibody
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