Anti-Rac1 Antibody (A15853)

Name: Anti-Rac1 Antibody
Brand: Antibodies.com
SKU: A15853
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Rac1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Rac1 Antibody

Description

Rabbit polyclonal antibody to Rac1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human Rac1/2/3 (NP_008839.2).

Sequence

MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cell migration inducing gene 5 protein, Cell migration-inducing gene 5 protein, MGC111543, MIG5, Migration inducing gene 5, Migration inducing protein 5, p21 Rac1, p21-Rac1, Rac 1, RAC1_HUMAN, Ras like protein TC25, Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1), Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1, Rho family small GTP binding protein Rac1, TC 25, TC25

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Rac1 Antibody (A15853)

Western blot analysis of extracts of various cell lines, using Anti-Rac1 Antibody (A15853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Rac1 Antibody (A15853)

Western blot analysis of extracts of A-431 cells, using Anti-Rac1 Antibody (A15853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Rac1 Antibody (A15853)

Immunohistochemistry analysis of paraffin-embedded human gastric using Anti-Rac1 Antibody (A15853) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Rac1 Antibody (A15853)

Immunofluorescence analysis of C6 cells using Anti-Rac1 Antibody (A15853) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).