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Recombinant Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274)

Western Blot - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121341) - Antibodies.com ELISA - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121274) - Antibodies.com ELISA - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121341) - Antibodies.com
$525

Recombinant goat monoclonal [RMG02] antibody to Rabbit IgG Fc (Biotin) for IP, ICC, IHC, Flow Cytometry, ELISA and WB (non-reduced).

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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+1 (314) 370-6046
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Name
Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin)
Description
Recombinant goat monoclonal [RMG02] antibody to Rabbit IgG Fc (Biotin).
Host Species
Goat
Target Species
Rabbit
Specificity
This antibody recognizes the Fc region of rabbit IgG.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced)
Recommended Dilutions
ELISA: 0.01-0.5 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml
Immunogen
Rabbit IgG.
Antigen Species
Rabbit
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein G chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121341) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274)
Western blot of non-reduced (-) and reduced (+) rabbit IgG (20ng/lane), using 0.2µg/ml of Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin). This antibody reacts to non-reduced rabbit IgG (~150 kDa).
ELISA - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121274) - Antibodies.comEnlarge Image
Figure 2: Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274)
ELISA of IgGs from different species shows Anti-Rabbit IgG Fc Antibody [RMG02] reacts to the Fc region of rabbit IgG. There is no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Rabbit IgG Fc Antibody [RMG02] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
ELISA - Anti-Rabbit IgG Fc Antibody (Biotin) [RMG02] (A121341) - Antibodies.comEnlarge Image
Figure 3: Anti-Rabbit IgG Fc Antibody [RMG02] (Biotin) (A121274)
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Anti-Rabbit IgG Fc Antibody [RMG02] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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