Immunofluorescence analysis of U-2 OS cells, using Anti-LMNB1 Antibody as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C. Secondary antibody: Anti-Rabbit IgG (H+L) (AF594) (AS074) at 1:100 dilution. Blue: DAPI for nuclear staining.