Rabbit F(ab')2 Anti-Rat IgG Antibody (HRP) (A282608)

$350

Rabbit F(ab')2 anti-rat IgG (HRP) secondary antibody for ELISA, IHC-Fr, IHC-P and IHC-R.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Rabbit F(ab')2 Anti-Rat IgG Antibody (HRP)
Description
Rabbit F(ab')2 anti-rat IgG (HRP) secondary antibody.
Host Species
Rabbit
Target Species
Rat, Mouse
Specificity
This antibody recognises Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
Applications
ELISA, IHC-Fr, IHC-P, IHC-R
Recommended Dilutions
ELISA: 1:500 - 1:1,000, IHC-Fr: 1:50, IHC-P: 1:50, IHC-R: 1:50
Immunogen
Purified rat immunoglobulin G.
Clonality
Polyclonal
Isotype
IgG
Conjugate

HRP

Product Form
Liquid
Concentration
1 mg/ml
Purification
Affinity chromatography of serum.
Formulation
Supplied in Phosphate Buffered Saline with 0.01% Thiomersal.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Horseradish peroxidase conjugated Rabbit F(ab')2 anti Rat IgG antibody recognizes Rat immunoglobulin G. Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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