This antibody is specific for RAB5A. It does not recognize RAB5B and RAB5C.
Applications
WB, IF
Dilutions
WB: 1:250-1:5,000, IF: 1:50-1:250
Reactivity
Human, Rat, Mouse, Canine, Monkey
Immunogen
Recombinant peptide derived from within residues a.a. 115 to the C terminus of mouse RAB5A, expressed in and purified from E. coli.
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Concentration
4 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
RAB 5, RAB 5A, Rab5, RAB5A member RAS oncogene family, RAB5A_HUMAN, RAS associated protein RAB5A, Ras related protein Rab 5A, Ras-related protein Rab-5A
Figure 1: Western Blot - Anti-RAB5A Antibody (A121551)
Endogenous RAB5A detected with Anti-RAB5A Antibody at a 1:2,500 dilution. Lysates at 50µg per lane and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.
Figure 3: Western Blot - Anti-RAB5A Antibody (A121551)
293 cells transfected with GFP-RAB5A, GFP-RAB5B, and GFP-RAB5C, detected with Anti-RAB5A Antibody at a 1:500 dilution. Rabbit anti-goat IgG antibody (HRP) was used at a 1:10,000 dilution.
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